To: ³BioPEEP² From: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² Subject: Progress report 11/3/95 Date: 11/3/95 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- The initial population of 254,423 chickens has now been tested. The 500 test apparatus are functioning well now that the flowcontrol valve has been modified to accommodate the acidic samples. Modifications have been made to the shrouding on the dataloggers to cope with the dusty environment. Current work is centred around packing the blood samples ready for their transfer to Santa Fe. We have not yet received the chicken breeding cages necessary for stage 2. Anthony Barsha Head Researcher, Enhanced Poultry Production, QLD, Australia. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNId9IhwfqXdTDGiZAQHh+wIAipAz+IcOI1KLbmpeN/0z4JAi6wQ7EyKI vLSizPpmyWZPwkG8WLTyEhxWi1Mhoo5KFL9vUPLvEdEoV0LxoMX83Q== =pVQp -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Error in data received Date: 11/23/95 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- To Head of Research: We have found an inconsistency in the last data ( 11/22/95) received: A6-55M/LJ The cage reference and chicken ID are inconsistent. Our version of the database gives: A6-55J/LJ 393926 A6-55K/LJ 100165 A6-55L/LJ 206478 A6-55M/LJ 313692 <=== A6-55N/LJ 383165 A6-55O/LJ 306248 Which is the correct entry? Vladimir Alkalaj Head of National Supercomputing Centre -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBUAwUBNIdjOHe4cIECyqttAQEkvQH3f2MuxZMjYJaUIe7TPmdgcBzoSzzYWtvs OJCST6k8tHhZyqUBDipJ1mPIqi3wijJDXzEVHh40GsMgIqBCggaM =k9uE -----END PGP SIGNATURE----- To: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" From: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² Subject: Missing data Date: 12/4/95 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Vladimir, The board on that test apparatus is working fine. We've just checked it. From our records we have the missing data. The voltages must have gone missing in transit from here to you. We'll resend Chicken ID 313692 and the next ten entries. Keeping us on our toes! Anthony Barsha Head Researcher, Enhanced Poultry Production, QLD, Australia. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNId+33e4cIECyqttAQE5jQIAoLDAOUVIH11mUfb23Xq5p4AbVqAp3AVH 8zKKM6ljj0MaXmVBBce1RlLqsslBj4HaG/svnQhalzgr6hI5CmuIDQ== =qFB8 -----END PGP SIGNATURE----- To: "Dr. Jason. S. Aaron, Director, Genscan" From: ³BioPEEP² Subject: Date: 1/13/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Dr. Aaron: I hope I don't have to continually re-emphasize the importance of secrecy within your company. This has become a strictly a don't ask, don't tell operation, as I have made clear before. In that regard, I will not be able to respond to your recent request. Again, I want to lay down our guidelines. Please make sure your employees understand them. 1) The various elements of this project are not to be discussed with anyone who is not known to be a member of the project. 2) Researchers are not to be offered any information beyond what is necessary for them to proceed with their research. 3) Researchers from the various sections are not to discuss their work with researchers outside their section. 4) All communications are to be done from outside of the complex. No packages or letters will be sent from or to the complex. This includes food delivery. 5) All safety and security procedures are to be rigorously followed. Failure to follow such procedures by any researcher or project member is grounds for instant dismissal. Please read the above and make certain that they are strictly enforced. BioPEEP -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeAuFiwY/xYnYZZAQG+rwIAmUPQDd9WDjQtTwZHPiEDb2f/9QlhHJHB RLtmbhPWdaAKmxcaqqRlon/CfCf+VelSGba7x6KLfaIkxXtbl7XZQw== =lXqs -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Genotyping progress Date: 2/6/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Complete genotyping database for first generation of addiction positive chickens was received from Santa Fe yesterday. All 447 microsatellites have been examined using the new robotic PCR sequencing technology GenScan have implemented. The 413 chickens, with an addiction correlate over 0.73, are to be targeted for further breeding. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeBZliwY/xYnYZZAQEhBwH6Ah5hWez4yF1yDBpJEY9C0RV3JCR78DRw eGX7Vhx8kQM/+XROcL5gYwHKqvEYcZAIriFdenJwuSValv1+s5/0zg== =BIDF -----END PGP SIGNATURE----- To: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: Segmentation Violations Date: 2/12/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Dear Dr. Alkalaj, I've just batched the next set of data into Fastlink. We've encountered some segmentation violations when running our larger datasets. We're thinking that there may be something going awry with the way the disks are mounted. At present the temporary files are placed into /usr/fast/temp/ and the output goes to /usr/fast/people/BioPEEP These segmentation violations are causing us some serious difficulties. We would also like to install a new package that has been developed especially for this project. We'd like to compile it for massive parallelism. Its been agreed that you need to know how this software works if you are to help us install it. We have used a genetic algorithm based method to optimize the fine scale loci ordering. This means we can order the 896 markers, which span the chicken genome, in under 4 days of CPU time. The GA based approach lends itself to massive parallel computation, not possible with other methods. We will require a considerable portion of your entire machine to realise the time deadline for this work. We thank you for your confidentiality in this matter. Dr. Aaron ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeCB1iwY/xYnYZZAQEmQgIAq74ZUeoDMqKxE5VskTWPgWs2XXoleBNW nDxg1j83q3PN0rKJ7QRBGj9QaYRulDQVpGh84aqK3llGxGhUJ+o06Q== =Bush -----END PGP SIGNATURE----- To: "BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Avian influenza virus Date: 6/1/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- We have had some trouble. Our best efforts to contain the outbreak has gone awry, luckily it was reported as a natural outbreak of Avian Influenza (AP 5/31/97). > The shipment of the new avian influenza virus was involved in a car > accident in Harrisburg, Pennsylvania. An isolation team > decontaminated the crash site and removed all evidence of the > accident. We are worried the large accident area and contagious > nature of the virus may mean uncontrolled outbreaks of the H7N2 > strain. We suggest culling all nearby flocks as a preventative > measure. > > > Miyamoto Togakure > PC4 Containment Facility > ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIjGWRwfqXdTDGiZAQFAVQH/eSb/t2po97IuQ9UXTF8X0KhZZQUlm1z0 ycexQgAvZehpAHC41R9adNxNVgWYzND2Zz3UlmeBl68Q3XLAfkNa4A== =tqE3 -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Warren Dastrup" Subject: Progress Report 3/6/96 Date: 3/6/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- I apologize for the delay in communication. It has taken us a while to get up to speed, as the project is quite an ambitious one. Things are moving along, however, with the collection and processing of the data down the road. If things continue to proceed smoothly at the New Mexico end, it shouldn't be long before we are able to transform our data into the materials you have requested for your experimentation. Some additional clarification of the research parameters would be especially helpful at this time, as the Santa Fe techs are constricted to such a tight time frame. Some questions: What final form will the bundles take? Will there be several different forms (i.e. cola, toothpaste, etc.) At what dosage? Will the dosage remain constant through the experimentation? If your plan is now fully developed, please give me a clear sense as to how you envision your experiments proceeding so as to save as much time/effort as possible. With great regard, Warren Dastrup Director of Research Pacific Research Associates PO Box 1682 Kapaa, HI 96746 -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIdmJhMbdvJfVmuxAQFe6gH/b2cpFn9BvkXsfvOE/y5dazDAQsDtCiIM QZgwTfHRqrESMn2PnOb3vQZUZ5Xq8r3NMNIjb0sVwM2KYlua9sf5LA== =Do8u -----END PGP SIGNATURE----- To: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" From: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² Subject: Testing complete Date: 6/1/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Psychological testing of the second generation of chickens is now complete. We have placed the data on our ftp server encrypted in the usual manner. The genotyping should be completed very soon as it has been 3 months since we sent the shell tissue samples to Santa Fe. Santa Fe has been having difficulty with automating DNA extraction of this tissue and this may delay their data to you. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeDVhwfqXdTDGiZAQHSfwH9EU+QWThlHJHTQBXrA9xzNMR3U9yjD6nK OOwUi8ZzIwomGoj3kk4JF2RoJPQ/crE+P0W+aBk7WcCWH1pcSgLAdA== =Ftvi -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Success of experimental crosses Date: 10/11/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- We have received third generation chicken psychological tests. Two thirds of genotype data is still to come from Santa Fe. Preliminary results show addictive chicken crosses have produced high addiction correlates. Chi squared test gives certainty of 99.8% that single loci are involved in at least some of results. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeD2BMbdvJfVmuxAQGMEgH7BGcRluFHl6hrUBNt2+rB53Ve12DsShTl nr3FfCS/hOpNmd2VX1/50nJ2myGg/uJhHG5Z2/7GyRmtdWllJXYCSQ== =zsPe -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: 3rd Gen Genotping data complete Date: 12/6/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- All genotype data for third generation is complete. We are running CEPH linkage analysis on Convex supercomputer. New parallel implementation will reduce computation time to within 1 month. Addiction correlate phenotype bottleneck has been resolved by using a Baysian model of data, which assumes genetic diversity of chicken population. We are hopeful of an outcome within 1 month. -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeEV26iSGWlgnuhAQFJsgIA1e821p/UpmKkC/MBbZQlN0NQHzZ4UM2b CL3Cu2hvSFCdjcK6KkzsYCiY9up6PhnimUEN7UoWNmBV1DJ8TWaHOA== =uY6P -----END PGP SIGNATURE----- To: "Dr. Jason. S. Aaron, Director, Genscan" From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Localisation of positive loci Date: 12/18/96 Cc: Bcc: X-Attachments: CEPH_family_432201.jpg -----BEGIN PGP SIGNED MESSAGE----- We have located a positive addiction gene using linkage analysis on the family 432201 and related pedigrees. This red jungle fowl, hen_432201, has a positive loci between microsatellites MCW0095 and MCW0158 (East Lansing). We are unable to specify the exact area between these markers where the loci occurs. We are having trouble with repeatability with Komsabi map length in this region of chromosome 8. Regards, Vladimir Alkalaj -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeFLHe4cIECyqttAQGrtQH+JytRg5/9A7MRTqZVzaS3ArrYsn0dD+16 xJy0+xzijBz+MPKc8KHurMel/nLxxqx9Qg0GiDH9Sg86J5WSHDcgJg== =mF+t -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: Discovery of an addiction locus Date: 12/23/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Linkage analysis has uncovered an addiction gene somewhere distal to microsatellite MCW0095 (mapping KIT 3). We have hybridized YACs covering MCW0095 to MCW0158 (the region of interest). Using a chick brain (14 day embryo) cDNA library we have uncovered one YAC which is a prime contender for an addiction locus. The cDNA library is in a pBR322 vector (X1776 E.coli) using the HindIII cloning site. A rapid sequencing strategy of the whole MCW0095-MCW0158 region using r andom hexamer walking has produced a 3.7 Mb contig which is currently being analysed for open reading frames. We soon hope to clone the MCW0095-MCW0158 addiction locus (Slovenian reference, locus=xp234). ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeFp1iwY/xYnYZZAQGwRAH/axt/S7FiMdy5trqpRG9y4fT4ezPLMuqe R603/Ux+yrBg6ANjSGg7I5hHkJz/rwJ1h6aeQFlb4E9eS+9sXQ+Sog== =XioE -----END PGP SIGNATURE----- To: ³BioPEEP² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Addiction gene located to YAC_37168C Date: 12/30/96 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- We have isolated the addiction locus in the region MCW0095-MCW0158 using several gene finding algorithms. YAC_37168C (697kb) contains a gene with homology to human D2 dopamine receptor. This gene, XP234, has multiple ORFs and is probably alternate spliced. We have emailed the map locating the gene to the transgenic facility. Best Regards, Vladimir Alkalaj -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeGIxwfqXdTDGiZAQEYnQH/a6vMLMuE9Fs98zAZt/2QpVeDTbfZr0Mg MZfQI5GSOut9GgqgPhq0u9eESVeKzeHW/eW3/3kI4+ih9+WHieXJgQ== =MmsY -----END PGP SIGNATURE----- To: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: re: Current protocols Date: 1/3/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Yes, we have constructed an expression vector for the XP234 gene and produced the antibodies. In-situ hybridization of chick brain has shown that XP234 is expressed in the nucleus accumbens, ventral pallidum and ventral tegmental areas. Considering these regions of the brain are associated with inbuilt reward systems and uncontrollable addiction-like physiologies, it is highly likely that the mutation in the promoter region of XP234 upregulates production of the chick D2 dopamine transmembrane receptor in the post-synaptic neurons. This would increase the protein phosphorylation and hence increase firing of the post-synaptic neurons. The physiological outcome being massive uncontrollable cravings similar to heroin addicts suffering withdrawal. We are highly confident of creating a successful addiction inducing vector in the very near future. ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIdorG6iSGWlgnuhAQGApwH+O4kzTWVvXiOKrMU3l8OW4OBoMa94LPMl I5gsuOOEDBlTGG0/BgbEsQdVuHJ1c4fGpCOjDFlo0OJg1EnT/7gEBQ== =PQi2 -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Possible function of the XP234 gene Date: 1/14/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- As per your request following is an executive summary of the current state of neurophysiological research apropos addiction with respect to the polymorphism in the promoter region of XP234: > I refer to your request for information regarding likely patho- > physiological effects of the proposed insertion of the addiction locus > (confidential mail, dated 11/27/96). My colleagues and I have > discussed the possible outcomes of the most likely primary effect of > the mutation, in a promoter for the D2 dopamine receptor in the > motivation and reward centres of the ventral striatum. > > Allow me to outline what we consider to be the most likely > mechanism of action for the up-regulation of the D2 signaling > pathway. > > As you will be aware, current research concerning the actions of the > reward and motivation pathways of the brain, particularly in relation > to addiction, stresses the importance of the dopaminergic > mesocorticolimbic projection from midbrain nucleii like the ventral > tegmental area and substantia nigra to diverse limbic and behavioral > centres in the ventral striatum, the best characterized centre of > which in relation to addiction remains the nucleus accumbens. This > projection is topologically diverse, and appears to mediate different > actions at a range of physiological levels at the site of projection. For > example, it has become increasingly obvious in recent years that the > nucleus accumbens, in keeping with what we already know about the > motor components of the nigro-striatal projection, is not a > homogenous population of targeted cholinergic neurons, but instead > encompasses a cytoarchitecturally diverse and functionally organized > neuronal microarchitecture. Hence it is now speculated that the > tegmental-accumbes projection is segregated at at least the level of > accumbens cytoarchitecture, a distinction between targeted > populations in matrix and shell areas now becoming anatomically > possible and physiologically differentiated. Secondly, a distinction > can be made at the level of the dopamine receptor. It now appears > that populations of D1 and D2 receptors are co-localized with known > cytoarchitecturally distinguished functional groups. Additionally, as > advances in molecular cell biology have allowed us to unravel the > signalling pathways of the relevant receptors, this information has > been increasingly compared with known behavioural data to form an > integrated picture of the addiction and reward brain pathways. > > As your proposed insertion would primarily lead to an increase in > the D2 receptor population density in the accumbens and olfactory > tubercle, it seems likely that any activity of the dopaminergic > projections to these sites would elicit an enhanced physiological > response. There are several lines of investigation from parallel > disciplines, including pharmacological and reward centre activation > paradigms, which allow us to suggest homologous models of the > physiological modulation you propose. Pharmacological studies of > strongly addictive drugs like cocaine, which both directly stimulates > dopamine release and prevents dopamine reuptake, suggest that > systemic application of these drugs will lead, via physiological > mechanisms that have been strongly likened to classical > conditioning-like Hebbian strengthening, to a strong pleasurable > association with particular behavioral situations and the particular > drug of abuse. Reward center activation, in the current literature, in > which the animal directly stimulates it's own reward centers via an > intra-cranial micro-electrode connected to a level-press, offer further > circumstantial evidence to support the prediction that your insertion > should promote strong addiction-like behavioural responses by > administered rats. The pattern of behaviour associated with > administration is probably also likely to include addiction-like > responses to the associated addiction vehicle (in humans, for > example, the needle) like craving, sympathetic activation, etc. > > We predict, in other words, strong addiction and craving behaviours to > be expressed towards the vehicle of administration, particularly > where this vehicle is demonstrated to be the vehicle of > administration (ie. by training) to the animal. > > In summary, we also believe that the proposed mutation offers a > excellent research vehicle for the study of D2-receptor-mediated > addiction effects, particularly as they relate to the presentation of > novel, yet addictive, drug-administration vehicles. > > We intend this letter only as an executive summary; our detailed > report is available on request. > > Yours Sincerely, > > S. Nakamura > > ================================ > Shigeru Nakamura > Senior Research Fellow > Cognitive Neuroscience Unit > Genscan U.S. > PO Box 28754 > Santa Fe, NM 87592-8724 > ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeHCBMbdvJfVmuxAQHvVQH8Cbwqrut7kKVuHHKDlbul5xVlQz6Fln1O VbweKhMQCA0vzpVuIRMNxYeBueBO/jyAOSroDZ2eu+Jb2fxeXeJg1g== =Q4BN -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: 9 Positive single loci addiction phenotypes Date: 1/20/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Complete linkage has given 9 single loci in 15 CEPH families with LOD scores clustered within the range 5 to 6. Other positives were either complex, incomplete penetrance or multi-gene phenotypes which would require further analysis and more CEPH data. I am confident that the 9 loci will be placed physically with an accuracy of greater than 700 Kb on the physical map. Currently recombination distance to physical distance for our poultry is 1 centiMorgan per 527 kb. With the addition of the new markers and extra allelic information it is now possible for us to place the positive loci to within an accuracy of 0.5 cM. Vladimir Alkalaj Head of National Supercomputing Centre -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeHoW6iSGWlgnuhAQHT/AH+OemhjiBHPPONx3iDF7ZemnM6iD5G1iWx jjPdREoc3uV69gozRSj7IPt6BdJ8Y1mahvPvxtzjMfejc+XJeDnzLQ== =JFN4 -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: Current status of gene isolation Date: 2/14/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- The chicken YAC library clones containing all 9 positive loci have been sent to us as frozen glycerol stocks from an outside source. They are completely unaware as to our use of these clones. We have already digested and isolated fragments which hybridize to both microsatellites for each positive locus. The next step has been started. We decided on a primer walking strategy to minimise any time delays. An order has been placed for another oligosynth to cope with the workload. Please verify payment to speed this process. ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIebRRwfqXdTDGiZAQGpTQIAhjRMGtvXWWIiNTW8JXmSqy5nTHn6YiVB +8/bASPZUqDNdvoNtOJDsgc985nDpxmwPL1WDcGqcU2UqBpVclvHGQ== =/v0M -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: Status of YAC sequencing. Date: 3/2/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Genscan has started the multiplex primer walking. The 9 fragments containing the positive loci have been sequenced in record time. GenScan will place the 9 contigs on our Santa Fe server for the Slovenian facility to upload later this evening. We will send you more specific information as to the next round of breeding within the week. ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIebt26iSGWlgnuhAQEpQwIAyfrQYUmH3a1jRkMixU7UJ75Vs71367vI W23mVlQwPDBM8EOCgriR+hE4/2Wt3I96fJstDffQTD7tODzBVESBZw== =kGzm -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "V.Alkalaj,IJS-NSC,+386 61 1773900, 1773666" Subject: Update on bioinformatic analysis of YAC contigs Date: 3/8/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- One of the nine contigs contains no ORF. This may mean that there is some unknown control element or an important enhancer in this region or it may be that this locus was false positive (LOD score 5.3). We believe latter as locus was placed in experiment even though LOD score was below significance. 8 other contigs have genes. One contig has 3 genes. 5 contigs have 2 genes. 2 contigs have 1 gene. 9 of 15 genes have no homology to known sequences in Genbank. Other genes have homology to tyrosine kinase receptor, neural cell adhesion receptor, transcription factor, vasopressin receptor, ribonucleoprotein, insulin-like factor receptor, D2 dopamine receptor and insulin protein. Vladimir Alkalaj Head of National Supercomputing Centre -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIeb/3e4cIECyqttAQFNPwH/YEDxpaQJD3EL3lCHmGBgE+amsdu9G55C 4tCvSL+uSQBTeajF0pHJsU9v2LrKDD1MX2COKPoy6oWpUZrMc0Fhbw== =3+jy -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: 246 transgenic chicks Date: 8/4/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- 14 genes were subcloned from YAC library clones. We then inserted the plasmids into fertilised chicken embryos and used a neomycin selection protocol. Our new technique allows us to breed chimaera chickens from unfertilized eggs injected with stem cells. The success rate is low but tolerable. We will be sending the chickens to Australia for psychological evaluation by the 24th. ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIecNne4cIECyqttAQF+kQIAw3LkcZtoAgWyBqAVYuU27VPzFbEySGYb PVF1TSILWyAo3YuamyOEXE5kuPRa+c2CPCZWnz48dFnUf2I+K+PTOA== =KC8+ -----END PGP SIGNATURE----- To: "Anthony Barsha, Head Researcher, Enhanced Poultry Production, QLD² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Avian influenza virus vector Date: 9/12/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- > > The avian influenza (H7N2 strain) virus vector, containing all 5 genes > shown to cause addictive behaviour in transgenic chickens, has been > constructed. It is a high virulence vector capable of selectively > infecting neural and other non-dividing tissue. It is quickly > integrated into the genome as PCR has demonstrated and is capable > of lying dormant for a prolonged period. Viral promoters have been > attached to the 5 addiction genes, which has caused a tremendous > increase in their power. The test apparatus arrived last week from > Australia and we have verified that chickens infected by intra- > cranial injection with the virus have an addiction correlate of 0.98. > > Miyamoto Togakure > PC4 Containment Facility > ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIee8RMbdvJfVmuxAQHjAAH/TqcovUqNqZbCOyHUWt20pdjiEmEDacfE KyecHsoOUvcdnAkJcj1oAJ0892qTbJPZ5qvyS5nJlFfacwfFpKKGsw== =BaGM -----END PGP SIGNATURE----- To: ³BioPEEP² From:"Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Blood-brain permeabliser Date: 9/15/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- > > The new blood-brain permeabilising drug is highly effective. The > avian influenza virus now passes quickly into the brain causing > chickens ingesting the virus-drug-cola mixture to become extremely > addictive within 18 hours of ingestion. To transport the virus > across the blood-brain barrier we have used a novel nanometer sized > delivery system based upon a magnetic iron crystal coated in > selected organic compounds mimicing the cell membrane. The viral > protein coat has been modified to bind to the iron cored partisome > and also mimic the MDCK cell membrane. The potential of this > delivery system is enormous. The behaviour altering properties of a > viral/partisome delivery system could be of great interest to military > groups as a highly lethal biological weapon system. > > Miyamoto Togakure > PC4 Containment Facility > ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIefPhwfqXdTDGiZAQHEOAIAhOxTeDD86CWKNYAImq2eSBIwxXvoMveQ /hU4xNpI0qMklsDQIDuROw5V30AvNKJZrtmc+fAnZaLD/pGIyJVVKg== =b9M4 -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Infectious Viral Vector Date: 10/18/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- > > The new virus, highly infectious and capable of passing across the > blood-brain barrier unassisted, has passed initial tests. Inocculated > chickens infect other chickens by contact. Chickens infected this way > become addictive within 36 hours of contact with an infected > chicken. Considering the Harrisburg Pennsylvania outbreak earlier > this year, a chance infection of a native chicken population could > have disastrous consequences considering the pathological behaviour > associated with the high addiction correlate. > > Miyamoto Togakure > PC4 Containment Facility > ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIefhliwY/xYnYZZAQGfxAIAj/Uv3I838fBLT+XTul6+7zWvuJbU/xMH NR+2ga1dqLyb9P0Z05x5OH+K+DBlSL2oWuR8+xgaCNyg398qX/AVRw== =5ri0 -----END PGP SIGNATURE----- To: ³BioPEEP² From: "Dr. Jason. S. Aaron, Director, Genscan" Subject: fwd: Human tests of the Herpes virus vector Date: 11/3/97 Cc: Bcc: X-Attachments: -----BEGIN PGP SIGNED MESSAGE----- Following is a brief update consistent with your recent request for weekly reports, outlining the progress of the human vector: > > The human homologs of the 5 addictive genes have been placed in a > modified HSV viral vector. Infection is by innoculation, however it > has been shown in the Borneo population that a large viral load > taken orally is also effective. Grave risks are associated with the viral > promoters used in this construct. Insufficient research has been done > on the stability of integration into the genome. We are treating the > virus as an extreme carcinogen until further research can be carried > out into the integration stability of this vector. > Trial infections based in Asia show that the addictive behaviour > seen in chickens carries over to humans. We are unsure whether the > germ-line infection research is warranted considering the high > virulence of the Sydney Olympics strain created in this laboratory. > Further research is needed to stabilise the virus in its active > form in the acidic conditions it will be transported in. Current > experimentation uses sorbitol as a transfer agent dissolved in a > phosphoric acid buffer. The sorbitol could also be a selling point as a > natural sweetener without the calories of sugar. > ================================ Dr. J.S. Aaron, Director Genscan U.S. PO Box 28754 Santa Fe, NM 87592-8724 ================================ -----BEGIN PGP SIGNATURE----- Version: 2.6.3i Charset: noconv iQBVAwUBNIefwm6iSGWlgnuhAQH3PgH/bFhI8oAXxxp+XkZXkT8o8XoEz3CSzcaT KM9dGbg3ti9MG/2+erqM1gBG8vMSWZkvTRTstkSo4pKJmyY2fC4WPg== =BDcQ -----END PGP SIGNATURE-----